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Following primers were used to generate the W114A mutation: forward 5-GCTCTTTGAATTCGGGCTACCGAGTAG-3 and reverse 5-CTACTCGGTAGCCCGAATTCAAAGAGC-3, as well as forward 5-GCTCTTTGAATGCGGGCTACCGAGTAG-3 and reverse 5-CTACTCGGTAGCCCGCATTCAAAGAGC-3

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Derived from a compound found in the French lilac (goats rue), Metformin is now the most widely used oral medication for managing type 2 diabetes globally, often prescribed off-label for conditions like polycystic ovary syndrome (PCOS) and, in some cases, for weight loss