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2015) using small RNA sequencing analysis identified eight serum miRNAs (miR-122, miR-375, miR-423-5p, miR-30d-5p, miR-125b-5p, miR-4732-5p, miR-204-5p and miR-574-3p) which were increased more than twofold in APAP overdose samples ( n = 8)

RRID:Addgene_113569) (Watertown, MA, USA) and amplified according to the plasmid DNA Library Amplification protocol using 10-beta Electrocompetent E