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Though the group reported appreciable 1000:1 selectivity for hMC4R affinity compared to hMC1R affinity, functional cAMP assay results were only reported for hMC4R (EC 50 = 88 nM, E max = 85%), seen in Table 1 (55)

Reactive gliosis Spectrum of molecular, cellular and functional changes in glial cells, particularly astrocytes and microglia, that occur in response to central nervous system (CNS) injury, inflammation or neurodegeneration

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demonstrated that, relative to the ad libitum group, the CR group showed markedly higher levels of bone resorption markers (CTX and TRAP5b), accompanied by a notable decrease in the bone formation marker bone-specific alkaline phosphatase (BAP)